Experimental browser for the Atmosphere
If enough sample is present and the hydrophobic junk is not getting carried over, I would reinject a high on-column load, preferably 2x, to confirm the peptide availability in the samples.. If detergents are used in affinity pulldown, SP3 or S-trap would be ideal for sample prep .
Apr 6, 2025, 9:23 AM
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"text": "If enough sample is present and the hydrophobic junk is not getting carried over, I would reinject a high on-column load, preferably 2x, to confirm the peptide availability in the samples.. If detergents are used in affinity pulldown, SP3 or S-trap would be ideal for sample prep .",
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