Experimental browser for the Atmosphere
We developed a multiplexed CRISPR in vivo screening strategy (aka Perturb-seq) targeting genes expressed in the CLE and neural tube Key refinement: incorporating capture sequences into gRNAs to enable single-cell RNA-seq tracking of perturbations
Feb 13, 2025, 7:50 AM
{ "uri": "at://did:plc:n5osxqos2rlj4wpp2zw7qy4c/app.bsky.feed.post/3li27bz6hys2f", "cid": "bafyreid4m3mj5jqraokezq3bjfnz5hdjwnlwajt3wsbbvv23sezeiwdive", "value": { "text": "We developed a multiplexed CRISPR in vivo screening strategy (aka Perturb-seq) targeting genes expressed in the CLE and neural tube\n\nKey refinement: incorporating capture sequences into gRNAs to enable single-cell RNA-seq tracking of perturbations", "$type": "app.bsky.feed.post", "embed": { "$type": "app.bsky.embed.images", "images": [ { "alt": "", "image": { "$type": "blob", "ref": { "$link": "bafkreig6qbvpgm5em573aycddflupofsdurrthiffc4btzzar4ed57ujbu" }, "mimeType": "image/jpeg", "size": 70679 }, "aspectRatio": { "width": 787, "height": 199 } } ] }, "langs": [ "en" ], "reply": { "root": { "cid": "bafyreiflintzbs6fniysf63gm7uraurnm2xzcmu76dxr32pwfi4zvuxf3a", "uri": "at://did:plc:n5osxqos2rlj4wpp2zw7qy4c/app.bsky.feed.post/3li27bwdcz22f" }, "parent": { "cid": "bafyreiah66mvwryevm7viji654kwzkdvaboerjpkh3cnbyio5e6qpqi4iu", "uri": "at://did:plc:n5osxqos2rlj4wpp2zw7qy4c/app.bsky.feed.post/3li27bymxhc2f" } }, "createdAt": "2025-02-13T07:50:28.398Z" } }