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I done that with a multiple sequence alignment of representative target sequences and Geneious, but I am sure there are many ways to go about it! help.geneious.com/hc/en-us/art...
Mar 27, 2025, 1:53 AM
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"title": "How do I design degenerate primers based on an alignment?",
"description": "Using the Design New Primers operation\nGeneious Prime can design degenerate primers based on an alignment using the Design New primers tool under the Primers menu. For example, if you want a degen..."
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